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人宫颈癌细胞;Hela

更新时间:2016-06-15

简要描述:

人宫颈癌细胞;Hela,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。

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人宫颈癌细胞;Hela

人宫颈癌细胞;Hela
形态特性 上皮样
生长特性 贴壁生长 
特征特性 人宫颈癌细胞;HelaHeLa是*个来自人体组织经连续培养获得的非整倍体上皮样细胞系,它由Gey GO等在1951年从31岁女性黑人的宫颈癌组织建立。经原始组织切片重新观察,Jones等将其诊断为腺癌。已知该细胞系含有人乳头状瘤病毒HPV18序列,需在2级生物安全防护台操作。该细胞角蛋白阳性,p53表达量较低,但表达正常水平的pRB(视网膜母细胞瘤抑制因子)。
培养条件 RPMI 1640 (w/o Hepes) 10%FBS 
传代方法 1:2~1:6传代;2~3天1次。
传代情况 P130
冻存条件 基础培养基+5%DMSO+20%FBS 
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究。
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