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小鼠胚胎成纤维细胞;NIH/3T3

更新时间:2016-06-15

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小鼠胚胎成纤维细胞;NIH/3T3,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。

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小鼠胚胎成纤维细胞;NIH/3T3

小鼠胚胎成纤维细胞;NIH/3T3
形态特性 成纤维细胞样 
生长特性 贴壁生长 
特征特性小鼠胚胎成纤维细胞;NIH/3T3NIH/3T3是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株,建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞株对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感,对DNA转化及转染研究十分有用。鼠痘病毒阴性。
培养条件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS 
传代方法 当细胞生长至80%或80%以下汇合时即可传代,切勿达到汇合;1:3~1:6传代,每周2次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS 
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究。
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细胞外基质蛋白1(ECM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Extracellular Matrix Protein 1 (ECM1) X13860 48T/96T Mus musculus (Mouse)
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多胺调节因子1结合蛋白1(PMFBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) X13863 48T/96T Homo sapiens (Human)
载脂蛋白O(APOO)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein O (APOO) X13864 48T/96T Homo sapiens (Human)
纤维鞘相互作用蛋白1(FSIP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrous Sheath Interacting Protein 1 (FSIP1) X13865 48T/96T Homo sapiens (Human)
连接附着分子3(JAM3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Junctional Adhesion Molecule 3 (JAM3) X13866 48T/96T Homo sapiens (Human)
连接附着分子1(JAM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Junctional Adhesion Molecule 1 (JAM1) X13867 48T/96T Homo sapiens (Human)
中间丝蛋白(SYNC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Syncoilin (SYNC) X13868 48T/96T Homo sapiens (Human)
抵抗素样蛋白β(RETNLβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Resistin Like Beta (RETNLb) X13869 48T/96T Homo sapiens (Human)
毒蕈碱型胆碱受体M2(CHRM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2) X13870 48T/96T Mus musculus (Mouse)
毒蕈碱型胆碱受体M2(CHRM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2) X13871 48T/96T Rattus norvegicus (Rat)
胸腺基质淋巴细胞生成素(TSLP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thymic Stromal Lymphopoietin (TSLP) X13872 48T/96T Mus musculus (Mouse)
L1-细胞粘附分子(L1CAM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for L1-Cell Adhesion Molecule (L1CAM) X13873 48T/96T Mus musculus (Mouse)
天冬酰胺合成酶(ASNS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Asparagine Synthetase (ASNS) X13874 48T/96T Homo sapiens (Human)
白细胞弹性蛋白酶抑制因子(LEI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Leukocyte Elastase Inhibitor (LEI) X13875 48T/96T Mus musculus (Mouse)
蛋白酪氨酸磷酸酶受体G(PTPRG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Protein Tyrosine Phosphatase Receptor Type G (PTPRG) X13876 48T/96T Homo sapiens (Human) 兴奋性氨基酸转运蛋白1(EAAT1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Excitatory Amino Acid Transporter 1 (EAAT1) X13877 48T/96T Homo sapiens (Human)
驱动蛋白家族成员5A(KIF5A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Kinesin Family, Member 5A (KIF5A) X13878 48T/96T Homo sapiens (Human)
驱动蛋白家族成员18A(KIF18A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Kinesin Family, Member 18A (KIF18A) X13879 48T/96T Homo sapiens (Human)
G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER) X13880 48T/96T Homo sapiens (Human)
Bcl2相关髓细胞白血病序列1(MCL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) X13881 48T/96T Homo sapiens (Human)
IgG-Fc片段结合蛋白(FcγBP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fc Fragment Of IgG Binding Protein (FcgBP) X13882 48T/96T Homo sapiens (Human)
序列相似家族132成员A(FAM132A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Family With Sequence Similarity 132, Member A (FAM132A) X13883 48T/96T Homo sapiens (Human)
形成素结合蛋白1(FNBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Formin Binding Protein 1 (FNBP1) X13884 48T/96T Homo sapiens (Human)
肌脂蛋白(SLN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sarcolipin (SLN) X13885 48T/96T Homo sapiens (Human)
D-多巴色素变位酶(DDT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for D-Dopachrome Tautomerase (DDT) X13886 48T/96T Homo sapiens (Human)
归巢关联细胞黏附分子(HCAM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Homing Associated Cell Adhesion Molecule (HCAM) X13887 48T/96T Mus musculus (Mouse)
钙网蛋白(CRT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Calreticulin (CRT) X13888 48T/96T Rattus norvegicus (Rat)
钙腔蛋白(CALU)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Calumenin (CALU) X13889 48T/96T Rattus norvegicus (Rat)
成纤维细胞生长因子19(FGF19)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 19 (FGF19) X13890 48T/96T Rattus norvegicus (Rat)
S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) X13891 48T/96T Rattus norvegicus (Rat)
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Ⅶ型胶原(COL7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type VII (COL7) X13894 48T/96T Mus musculus (Mouse)
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泛素关联蛋白2(UBAP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ubiquitin Associated Protein 2 (UBAP2) X13897 48T/96T Homo sapiens (Human)
尿溶蛋白3A(UPK3A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Uroplakin 3A (UPK3A) X13898 48T/96T Homo sapiens (Human)
序列相似家族20成员A(FAM20A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Family With Sequence Similarity 20, Member A (FAM20A) X13899 48T/96T Homo sapiens (Human)
半胱氨酰白三烯受体1(CYSLTR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cysteinyl Leukotriene Receptor 1 (CYSLTR1) X13900 48T/96T Homo sapiens (Human)
弹性蛋白酶(ELA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Elastase 2A (ELA2A) X13901 48T/96T Rattus norvegicus (Rat)
碳分解代谢物阻遏4样蛋白(CCRN4L)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carbon Catabolite Repression 4 Like Protein (CCRN4L) X13902 48T/96T Homo sapiens (Human)
巯基丙酮酸硫转移酶(MPST)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mercaptopyruvate Sulfurtransferase (MPST) X13903 48T/96T Mus musculus (Mouse)
赖氨酰氧化酶样蛋白2(LOXL2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2) X13904 48T/96T Rattus norvegicus (Rat)
谷氨酰胺酶(GLS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glutaminase (GLS) X13905 48T/96T Homo sapiens (Human)
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糖磺基转移酶9(CHST9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carbohydrate Sulfotransferase 9 (CHST9) X13907 48T/96T Homo sapiens (Human)
微管关联蛋白4(MAP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Microtubule Associated Protein 4 (MAP4) X13908 48T/96T Rattus norvegicus (Rat)
双特异性磷酸酶1(DUSP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dual Specificity Phosphatase 1 (DUSP1) X13909 48T/96T Mus musculus (Mouse)
细胞色素P450家族成员1A2(CYP1A2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 1A2 (CYP1A2) X13910 48T/96T Rattus norvegicus (Rat)
细胞色素P450家族成员3A4(CYP3A4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 3A4 (CYP3A4) X13911 48T/96T Homo sapiens (Human)
δ样蛋白4(δLL4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Delta Like Protein 4 (dLL4) X13912 48T/96T Rattus norvegicus (Rat)
T-框蛋白3(TBX3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for T-Box Protein 3 (TBX3) X13913 48T/96T Homo sapiens (Human)
胃脂酶(LIPF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lipase, Gastric (LIPF) X13914 48T/96T Homo sapiens (Human)
粘蛋白2(MUC2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mucin 2 (MUC2) X13915 48T/96T Rattus norvegicus (Rat)
纤维蛋白原β(FGβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Beta (FGb) X13916 48T/96T Rattus norvegicus (Rat)
非神经元性烯醇化酶(NNE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Enolase, Non Neuronal (NNE) X13917 48T/96T Rattus norvegicus (Rat)
Ⅱ型胶原α1(COL2α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type II Alpha 1 (COL2a1) X13918 48T/96T Rattus norvegicus (Rat)
C-Mer原癌基因酪氨酸激酶(MERTK)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for C-Mer Proto Oncogene Tyrosine Kinase (MERTK) X13919 48T/96T Homo sapiens (Human)
Janus激酶3(JAK3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Janus Kinase 3 (JAK3) X13920 48T/96T Rattus norvegicus (Rat)
纤维蛋白原γ(FGγ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Gamma (FGg) X13921 48T/96T Rattus norvegicus (Rat)
前蛋白转化酶枯草溶菌素9(PCSK9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) X13922 48T/96T Mus musculus (Mouse)
心型脂肪酸结合蛋白(FABP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) X13923 48T/96T Sus scrofa; Porcine (Pig)
三碘甲状腺原氨酸(T3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Triiodothyronine (T3) X13924 48T/96T General
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Rho鸟嘌呤核苷酸交换因子7(ARHGEF7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) X13926 48T/96T Rattus norvegicus (Rat)
冠蛋白1A(CORO1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Coronin 1A (CORO1A) X13927 48T/96T Rattus norvegicus (Rat)
维生素D(VD)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vitamin D (VD) X13928 48T/96T General 含铜胺氧化酶3(AOC3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Amine Oxidase, Copper Containing 3 (AOC3) X13929 48T/96T Mus musculus (Mouse)
肽酰甘氨酸α酰胺化单氧酶(PAM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peptidylglycine Alpha Amidating Monooxygenase (PAM) X13930 48T/96T Homo sapiens (Human)
海藻糖酶(TREH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Trehalase (TREH) X13931 48T/96T Mus musculus (Mouse)
 

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