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小鼠睾丸畸胎瘤细胞;P19

更新时间:2016-06-17

简要描述:

小鼠睾丸畸胎瘤细胞;P19,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。

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小鼠睾丸畸胎瘤细胞;P19

小鼠睾丸畸胎瘤细胞;P19
形态特性 上皮样
生长特性 贴壁生长 
特征特性 小鼠睾丸畸胎瘤细胞;P19P19细胞株是从C3H/He小鼠中诱导的恶性畸胎瘤中建立的。该细胞在含有0.1mM 2-巯基乙醇的培养基中可高效率地克隆。该细胞具有多能性,在500nM 维A酸诱导下可以分化成神经和神经胶质样细胞;在0.5%~1.0%二甲亚砜(DMSO) 存在下,分化形成心脏和骨骼肌样细胞,但不形成神经或神经胶质样细胞;在DMSO和维A酸同时存在时,细胞的分化与只有维A酸一样。
培养条件 α-MEM:Alpha Minimum Essential Medium (with Nucleosides) 10%FBS 
传代方法 勿使细胞长至汇合状态,1:10传代;2~3天1次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS 
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究。
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