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人卵巢腺癌细胞;SK-OV-3

更新时间:2016-06-16

简要描述:

人卵巢腺癌细胞;SK-OV-3,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。

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人卵巢腺癌细胞;SK-OV-3

人卵巢腺癌细胞;SK-OV-3
形态特性 上皮样 
生长特性 贴壁生长
特征特性人卵巢腺癌细胞;SK-OV-3SK-OV-3由Trempe G和Old LJ在1973年从一位64岁白人女性卵巢腺癌患者的腹腔积液分离得到。该细胞对肿瘤坏死因子和几种细胞毒性药物(包括白喉毒素、顺铂和阿霉素)耐受。在裸鼠中成瘤,且形成与卵巢原位癌*的中度分化的腺癌。
培养条件 McCoy's 5A Media (Modified with Tricine) 10%FBS 
传代方法 1:2~1:6传代;每周2~3次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS 
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究。
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