更新时间:2016-06-15
小鼠骨髓瘤细胞;Sp2/0-Ag14,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。
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小鼠骨髓瘤细胞;Sp2/0-Ag14
形态特性 淋巴母细胞样;圆形
生长特性 悬浮生长
特征特性小鼠骨髓瘤细胞;Sp2/0-Ag14该细胞是由绵羊红细胞免疫的BALB/c小鼠脾细胞和P3X63Ag8骨髓瘤细胞融合得到的。该细胞不分泌免疫球蛋白,对20 μg/ml的8-氮鸟嘌呤有抗性,对HAT比较敏感;该细胞可以作为细胞融合时的B细胞组分用于制备杂交瘤;鼠痘病毒阴性。
培养条件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
传代方法 1:3传代;3~4天1次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究
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