更新时间:2016-06-16
人急性T淋巴细胞白血病细胞;Jurkat, Clone E6-1,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。
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人急性T淋巴细胞白血病细胞;Jurkat, Clone E6-1
形态特性 淋巴母细胞样
生长特性 悬浮生长
特征特性人急性T淋巴细胞白血病细胞;Jurkat, Clone E6-1该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。Jurkat细胞株来源于一个14岁男孩的外周血;经佛波酯和外源凝集素或抗T3单克隆抗体中的一种诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂);表达T细胞受体、CD3。
培养条件 RPMI 1640 (w/o Hepes) 10%FBS
传代方法 维持细胞浓度在1×105~1×106viable cells/ml;2~3天换液1次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究
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