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人小细胞肺癌细胞;NCI-H446

更新时间:2016-06-21

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人小细胞肺癌细胞;NCI-H446,开始培养保持细胞浓度在2×10 exp5个细胞/ ml和1×10 exp5和1×10 exp6个细胞/ ml之间。只用于实验室研究。不能被用于在临床检测和有关于人身安全的实验。

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人小细胞肺癌细胞;NCI-H446

人小细胞肺癌细胞;NCI-H446
形态特性 上皮样
生长特性 贴壁/悬浮生长,混合 
特征特性人小细胞肺癌细胞;NCI-H446该细胞是1982年由Carney D和Gazdar AF等从一位小细胞肺癌患者的胸腔积液中建立的。细胞的原始形态并不具有小细胞肺癌特征。这个细胞株是小细胞肺癌的生化和形态学上的变种,表达神经元*的烯醇酶和脑型肌酸激酶同工酶;左旋多巴脱羧酶、蚕素、抗利尿激素、催产素或胃泌激素释放肽未达到可检测水平。与正常细胞相比,该细胞c-myc DNA序列扩增约20倍,RNA增加15倍。zui初传代培养基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰岛素、0.01 mg/ml转铁
培养条件 RPMI 1640 (w/o Hepes) 10%FBS 
传代方法 1:3~1:9传代;每周换液2~3次。
传代情况 C5
冻存条件 基础培养基+5%DMSO+20%FBS 
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究
白介素27w(IL27w)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interleukin 27 Working Designation (IL27w) X17630 96T Rattus norvegicus (Rat)
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17631 96T Canis familiaris; Canine (Dog)
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粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17633 96T Mus musculus (Mouse)
肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17634 96T Bos taurus; Bovine (Cattle)
肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17635 96T Canis familiaris; Canine (Dog)
肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17636 96T Mus musculus (Mouse)
肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17637 96T Rattus norvegicus (Rat)
肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17638 96T Oryctolagus cuniculus (Rabbit)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17639 96T Bos taurus; Bovine (Cattle)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17640 96T Canis familiaris; Canine (Dog)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17641 96T Chicken (Gallus)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17642 96T Cavia (Guinea pig )
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17643 96T Sus scrofa; Porcine (Pig)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17644 96T Oryctolagus cuniculus (Rabbit)
干扰素γ(IFNγ)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17645 96T Rhesus monkey (Simian)
粒细胞集落刺激因子(GCSF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17646 96T Homo sapiens (Human)
粒细胞集落刺激因子(GCSF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17647 96T Mus musculus (Mouse)
粒细胞集落刺激因子(GCSF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17648 96T Rattus norvegicus (Rat)
胶质细胞系来源神经营养因子(GDNF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17649 96T Mus musculus (Mouse)
胶质细胞系来源神经营养因子(GDNF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17650 96T Rattus norvegicus (Rat)
核糖核酸酶T2(RNASET2)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17651 96T Homo sapiens (Human)
核糖核酸酶T2(RNASET2)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17652 96T Mus musculus (Mouse)
核糖核酸酶T2(RNASET2)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17653 96T Rattus norvegicus (Rat)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17654 96T Bos taurus; Bovine (Cattle)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17655 96T Capra hircus; Caprine (Goat)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17656 96T Equus caballus; Equine (Horse)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17657 96T Chicken (Gallus)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17658 96T Mus musculus (Mouse)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17659 96T Sus scrofa; Porcine (Pig)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17660 96T Rattus norvegicus (Rat)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17661 96T Oryctolagus cuniculus (Rabbit)
组织金属蛋白酶抑制因子3(TIMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17662 96T Rhesus monkey (Simian)
Rho鸟嘌呤核苷酸交换因子25(ARHGEF25)检测试剂盒(化学发光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17663 96T Homo sapiens (Human)
Rho鸟嘌呤核苷酸交换因子25(ARHGEF25)检测试剂盒(化学发光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17664 96T Mus musculus (Mouse)
Rho鸟嘌呤核苷酸交换因子25(ARHGEF25)检测试剂盒(化学发光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17665 96T Rattus norvegicus (Rat)
干扰素调节因子2结合蛋白1(IRF2BP1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17666 96T Homo sapiens (Human)
干扰素调节因子2结合蛋白1(IRF2BP1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17667 96T Mus musculus (Mouse)
干扰素调节因子2结合蛋白1(IRF2BP1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17668 96T Rattus norvegicus (Rat)
干扰素调节因子2结合蛋白2(IRF2BP2)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17669 96T Homo sapiens (Human)
干扰素调节因子2结合蛋白2(IRF2BP2)检测试剂盒(化学发光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17670 96T Mus musculus (Mouse)
成纤维细胞生长因子结合蛋白3(FGFBP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17671 96T Homo sapiens (Human)
成纤维细胞生长因子结合蛋白3(FGFBP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17672 96T Mus musculus (Mouse)
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血管内皮生长因子145(VEGF145)检测试剂盒(化学发光免疫分析法) CLIA Kit for Vascular Endothelial Growth Factor 145 (VEGF145) X17674 96T Homo sapiens (Human)
肝癌衍生生长因子样蛋白1(HDGFL1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17675 96T Homo sapiens (Human)
肝癌衍生生长因子样蛋白1(HDGFL1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17676 96T Mus musculus (Mouse)
肝癌衍生生长因子样蛋白1(HDGFL1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17677 96T Rattus norvegicus (Rat)
神经元衍生神经营养因子(NDNF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Neuron Derived Neurotrophic Factor (NDNF) X17678 96T Homo sapiens (Human)
锚定蛋白重复域蛋白50(ANKRD50)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17679 96T Homo sapiens (Human)
锚定蛋白重复域蛋白50(ANKRD50)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17680 96T Mus musculus (Mouse)
锚定蛋白重复域蛋白50(ANKRD50)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17681 96T Rattus norvegicus (Rat)
对氧磷酶1(PON1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Paraoxonase 1 (PON1) X17682 96T Homo sapiens (Human)
D-二聚体(D2D)检测试剂盒(化学发光免疫分析法) CLIA Kit for D-Dimer (D2D) X17683 96T Mus musculus (Mouse)
载脂蛋白A1(APOA1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Apolipoprotein A1 (APOA1) X17684 96T Mus musculus (Mouse)
肌原纤蛋白1(FBN1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Fibrillin 1 (FBN1) X17685 96T Homo sapiens (Human)
粘蛋白5B(MUC5B)检测试剂盒(化学发光免疫分析法) CLIA Kit for Mucin 5 Subtype B (MUC5B) X17686 96T Homo sapiens (Human)
Toll样受体4(TLR4)检测试剂盒(化学发光免疫分析法) CLIA Kit for Toll Like Receptor 4 (TLR4) X17687 96T Mus musculus (Mouse)
血清淀粉样P物质(SAP)检测试剂盒(化学发光免疫分析法) CLIA Kit for Serum Amyloid P Component (SAP) X17688 96T Homo sapiens (Human)
巨噬细胞清道夫受体1(MSR1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Macrophage Scavenger Receptor 1 (MSR1) X17689 96T Homo sapiens (Human)
主要碱性蛋白(MBP)检测试剂盒(化学发光免疫分析法) CLIA Kit for Major Basic Protein (MBP) X17690 96T Mus musculus (Mouse)
黑素瘤细胞黏附分子(MCAM)检测试剂盒(化学发光免疫分析法) CLIA Kit for Melanoma Cell Adhesion Molecule (MCAM) X17691 96T Mus musculus (Mouse)
载脂蛋白A4(APOA4)检测试剂盒(化学发光免疫分析法) CLIA Kit for Apolipoprotein A4 (APOA4) X17692 96T Homo sapiens (Human)
上皮型脂肪酸结合蛋白(FABP5)检测试剂盒(化学发光免疫分析法) CLIA Kit for Fatty Acid Binding Protein 5, Epidermal (FABP5) X17693 96T Homo sapiens (Human)
整合素β6(ITGβ6)检测试剂盒(化学发光免疫分析法) CLIA Kit for Integrin Beta 6 (ITGb6) X17694 96T Homo sapiens (Human)
膜联蛋白A1(ANXA1)检测试剂盒(化学发光免疫分析法) CLIA Kit for Annexin A1 (ANXA1) X17695 96T Homo sapiens (Human)
嘌呤能受体P2X7(P2RX7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) X17696 96T Homo sapiens (Human)
丝聚蛋白(FLG)检测试剂盒(化学发光免疫分析法) CLIA Kit for Filaggrin (FLG) X17697 96T Homo sapiens (Human)
 

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